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Создана первая в истории генетически модифицированная рептилия

Новости о технологии редактирования генома CRISPR появляются практически каждый день. Совсем недавно ученые использовали ее для внесения в одну клетку 13 000 изменений, а теперь впервые применили для редактирования генов рептилий. Первыми генетически модифицированными рептилиями стали ящерицы вида коричневый анолис (Anolis sagrei) — после изменения генов, они обрели бледно-розовый оттенок, и стали альбиносами.

Создана первая в истории генетически модифицированная рептилия

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В использовании метода CRISPR на рептилиях возникли некоторые проблемы — затруднения были связаны с особенностями их организмов. Обычно внедрение генетической конструкции производится практически сразу же после оплодотворения яйцеклетки, после чего изменения применяются на все другие клетки. К сожалению, в случае с рептилиями обычный подход был невозможен.

Дело в том, что самки многих рептилий, в том числе и ящериц, хранят сперматозоиды в своих яйцеводах длительное время, из-за чего момент оплодотворения уловить практически невозможно. Есть и другая проблема — оплодотворенная яйцеклетка покрывается оболочкой и не имеет внутреннего воздушного пространства, поэтому при введении инъекции сильно повышается риск повреждения эмбриона.

Создана первая в истории генетически модифицированная рептилия

Исследователи довольно легко обошли эти ограничения — они внедрили инъекцию в не оплодотворенные яйцеклетки. Редактированным геном стал фермент тирозиназа — при его инактивации, у животных возникает альбинизм. В общей сложности ученые ввели инъекции в 146 яиц, и в результате они смогли получить четыре бело-розовые ящерицы.

Новый метод предполагает, что в будущем исследователям удастся модифицировать гены и других рептилий. Какими именно особенностями они будут обладать, пока неизвестно.

Какие перспективы в новом открытии ученых вы видите? Своим мнением делитесь в комментариях, или же в нашем Telegram-чате.

Источник: news-ria.ru

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